Journal: Materials Today Bio

Article Title: Decellularized extracellular matrix scaffolds from Pleurotus ferulae mushrooms for sustainable production of steak-like cultured meat with authentic texture

doi: 10.1016/j.mtbio.2025.102327

Figure Lengend Snippet: Myogenic differentiation of bMuSCs and formation of cultured meat construct on a P . ferulae dECM scaffold. (A) Schematic illustrating the timeline for the proliferation and subsequent myogenic differentiation of bMuSCs on the scaffold. Created with BioRender.com . (B) Quantitative real-time polymerase chain reaction (qPCR) analysis of muscle-specific gene expression ( MYOG , MYOD1 and MYH7 ) in bMuSCs before and after differentiation on P . ferulae dECM scaffold. Data are presented as mean ± SD ( n = 3), with statistical significance determined by a Student's t -test (∗∗∗ p < 0.005). (C) Comparative histological analysis of the tissue structure with and without bMuSCs on P . ferulae dECM scaffolds by H&E staining. Scale bar = 200 μm. (D) Immunofluorescence images of bMuSCs after myogenic differentiation on P . ferulae dECM scaffolds, showing myotube formation via myogenic proteins Desmin (green) and MYF6 (red) expression. Nuclei are counterstained with DAPI (blue). Scale bar = 100 μm. (E) Quantification of the percentage of cells expressing Desmin and MYF6, respectively, relative to the total number of cells (DAPI-stained nuclei). (F) Fusion index calculated as the percentage of total nuclei residing within myotubes containing ≥2 nuclei. Data are presented as mean ± SD ( n = 3). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The primary antibodies used were an anti-desmin mouse monoclonal antibody (sc-23877; Santa Cruz Biotechnology, Dallas, TX, USA) and an anti-myogenic factor 6 (Myf-6) goat polyclonal antibody (NB100-1489; Novus Biologicals, Centennial, CO, USA).

Techniques: Cell Culture, Construct, Real-time Polymerase Chain Reaction, Gene Expression, Staining, Immunofluorescence, Expressing